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Spare Parts

Spare parts for NCBE kits

Spare Parts

Replacement parts for synthetic biology kit

Replacement parts for synthetic biology kit

Description

Replacement parts for synthetic biology kit:

  • 3 plasmids as in kit and transformation buffer (2 tubes of each plasmid and buffer)
  • Dried restriction enzymes + ligase as in kit (32 tubes of restriction enzymes and 16 tubes of ligase)

Plasmids needed for undertaking the synthetic biology practical and the dried restriction enzymes and ligase needed to cut the plasmids and then “stick” them back together.

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Gel tanks

Electrophoresis gel tanks (8)

£27.60

Description

Pack of eight NCBE electrophoresis gel tanks (without combs).

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Gel combs

Electrophoresis combs, 4-toothed (8)

£15.60

Description

Pack of eight gel combs for NCBE electrophoresis tanks (4-toothed).

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Gel combs

Electrophoresis combs, 6-toothed (8)

£15.60

Description

Pack of eight gel combs for NCBE electrophoresis tanks (6-toothed).

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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White tips

White tips (300)

£20.40

Description

Pack of 300 white tips for microsyringes (graduated at 2 and 10 microlitres).

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Carbon fibre

Carbon fibre electrode material (8 sheets)

£12.00

Description

Eight sheets of carbon fibre electrode material (100 mm x 100 mm) for NCBE gel electrophoresis tanks.

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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TBE buffer

TBE buffer For DNA electrophoresis 10x concentrate (50 mL)

£15.60

Description

50 mL of a 10x concentrate, which must be diluted before use (please refer to Teacher’s guide).

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Bromophenol blue loading dye

Bromophenol blue loading dye (8 x 1 mL)

£13.20

Description

8 x 1 mL tubes of bromophenol blue loading dye for DNA gel electrophoresis.

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Empty yellow tubes

Empty yellow tubes (16)

£11.40

Description

16 x 0.2 mL microtubes, yellow. For ‘The Lambda protocol’.

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Protein grade agarose

Protein electrophoresis grade agarose (6 g)

£20.76

Description

A fine grade agarose suitable for protein gel electrophoresis. 6 grams.

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Coomassie blue stain

Coomassie blue stain (200 mL)

£24.00

Description

An aqueous, colloidal, blue stain for protein gels. Unlike most Coomassie blue preparations, this does not contain methanol and is therefore safer for school use. 2 x 100 mL.

Note that salt must be added to this stain before use (please refer to Teacher’s guide).

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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TB buffer + SDS

TB buffer + SDS (10x 50 mL)

£19.20

Description

This concentrated buffer (10x 50 mL) is for protein electrophoresis. This buffer does not contain EDTA, to make it safer for school use. It is supplied with 3 mL of 10% SDS, which must be added to the diluted buffer before use (please refer to Teacher’s guide).

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Yellow non-graduated tips

Yellow, non-graduated 100 µL tips (100)

£11.40

Description

These are standard, 100 µL, non-graduated yellow tips, as supplied in the ‘Protein power!’ module.

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Microcentrifuge tubes

Microcentrifuge tubes 1.5 mL (100)

£12.00

Description

100 standard 1.5 mL Eppendorf type microcentrifuge tubes. Mixed colours.

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Filter films as supplied in kit

Filter films (6)

£17.64

Description

Replacement filter films for the ‘NCBE-SAPS Photosynthesis kit’. One each of red, green and blue, plus 3 different Neutral density (grey) filters. 13 cm x 20 cm.

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Transformer protocol p2k plasmid

Plasmid DNA in transformation buffer

£30.00

Description

Two tubes, each containing 2.1 mL of plasmid DNA, ready-diluted in transformation buffer, as supplied in the revised NCBE Bacterial transformation kit.

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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FTA cards

FTA cards for PCR kit (4)

£36.00

Description

Four plant FTA cards as supplied in the NCBE ‘PCR and plant evolution’ kit.

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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PCR primers + water

PCR Primers + Water

£18.00

Description

Replacement parts as supplied in the ‘PCR and plant evolution’ kit. Two primers plus sterile, ‘Nanopure’ water.

2 PCR primers + water … 1 tube of each

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Plasmids for ‘Nature’s dice’

Plasmids for ‘Nature’s Dice’

£52.80

Description

Three tubes of replacement plasmid DNA as supplied in the NCBE ‘Nature’s dice’ kit.

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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1 kb DNA Ruler

1 kilobase DNA ‘ruler’

£30.00

Description

A new version of the DNA ladder supplied in the NCBE ‘Nature’s dice’ kit and the plant evolution PCR kit. (130 µL of ready-diluted ladder, replacing the old ladder which you had to dilute yourself)

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Gel electrophoresis base unit

Gel electrophoresis base unit

£80.40

Description

The electrophoresis base unit contains eight sets of low-voltage electrophoresis equipment and dispensing units:

•8 NCBE gel tanks
•8 4-toothed combs
•8 6-toothed combs
•8 pairs of wires with crocodile clips
•8 microsyringe dispensing units (without tips)

https://www.ncbe.reading.ac.uk/electrophoresis-base-unit/

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Lambda DNA and enzymes pack

Lambda DNA and enzymes pack

£100.80

Description

16 tubes of each restriction enzyme (BamHI, HindII and EcoRI) and lambda DNA with 16 empty yellow tubes.

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Kanamycin and LB agar

Kanamycin and LB agar (2 sachets)

£27.72

Description

One sachet of kanamycin-containing and one of LB agar, as provided with the NCBE Bacterial transformation kit.

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Hydrogencarbonate indicator

Hydrogencarbonate indicator (250 mL)

£16.38

Description

10x concentrate of hydrogencarbonate indicator for use with the NCBE-SAPS Photosynthesis kit (250 mL).

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Electrode material

Carbon fibre electrode tissue for fuel cell

£11.40

Description

Carbon fibre electrode tissue for the NCBE Microbial fuel cell. (5 sheets, 10 cm x 22 cm)

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Ultraviolet keyring torch

Ultraviolet keyring torches (2)

£12.60

Description

Ultraviolet keyring-style torches, as provided with the NCBE Bacterial transformation kit (2).

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Microsyringes

Microsyringes (8)

£38.40

Description

Pack of eight microsyringes (to be used with graduated white tips).

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Kinetin solution

Kinetin solution, 1.25 mL

£8.82

Description

Solution of kinetin in 70% ethanol, as provided in the Cauliflower cloning kit.

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Culture vials

Vials for plant tissue culture (50)

£17.64

Description

50 empty culture vials as supplied in the Cauliflower culture kit.

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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MS medium + agar

MS medium + agar

£8.82

Description

Murashige and Skoog medium with vitamins and minerals plus agar powder. Sufficient for 500 mL of plant growth medium. As supplied in the NCBE Cauliflower cloning kit.

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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TE-1 buffer

TE-1 buffer

£7.20

Description

TE-1 buffer, 6 mL, as provided in the NCBE 'PCR and plant evolution' kit.

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Purification reagent

Purification reagent

£14.40

Description

Purification reagent, 6 mL, as provided in the NCBE 'PCR and plant evolution' kit.

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Micropipette 5uL

Fixed volume micropipette 5uL - Sorry, but we no longer supply this size

£27.60

Description

Unlike other cheap pipettes, these Volac 'MiniPipets' are accurate, autoclavable and robust. They even have a 'double action' like conventional micropipettes costing ten times as much. For more information, please click on the link below:

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Micropipette 10 uL

Fixed volume micropipette 10uL - Sorry, but we no longer supply this size

£25.20

Description

Unlike other cheap pipettes, these Volac 'MiniPipets' are accurate, autoclavable and robust. They even have a 'double action' like conventional micropipettes costing ten times as much. For more information, please click on the link below:

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Micropipette 20 uL

Fixed volume micropipette 20uL - Sorry, but we no longer supply this size

£27.60

Description

Unlike other cheap pipettes, these Volac 'MiniPipets' are accurate, autoclavable and robust. They even have a 'double action' like conventional micropipettes costing ten times as much. For more information, please click on the link below:

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Micropipette 50 uL

Fixed volume micropipette 50 uL

£28.80

Description

Unlike other cheap pipettes, these Volac 'MiniPipets' are accurate, autoclavable and robust. They even have a 'double action' like conventional micropipettes costing ten times as much. For more information, please click on the link below:

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Micropipette 25 uL

Fixed volume micropipette 25 uL

£28.80

Description

Unlike other cheap pipettes, these Volac 'MiniPipets' are accurate, autoclavable and robust. They even have a 'double action' like conventional micropipettes costing ten times as much. For more information, please click on the link below:

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Micropipette, 100 uL

Fixed volume micropipette 100 uL - Sorry, but we no longer supply this size

£27.60

Description

Unlike other cheap pipettes, these Volac 'MiniPipets' are accurate, autoclavable and robust. They even have a 'double action' like conventional micropipettes costing ten times as much. For more information, please click on the link below:

https://www.ncbe.reading.ac.uk/molecular-biology-replacement-parts/

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Replacement part for photosynthesis kit: 3 g enrichment medium

Replacement part for photosynthesis kit: 3 g enrichment medium

£13.86

Description

Enrichment media needed to grow the algae, this is enough for 2L of algae.

https://www.ncbe.reading.ac.uk/

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DNA tubes

Pre-digested DNA (5 tubes) as in kit

£40.80

Description

These are the replacement items for the DNA detective kit: the same 5 tubes of pre-digested DNA.
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DNA tubes with ruler

Pre-digested DNA (5 tubes) as in kit + 1 kb DNA ruler (ready-diluted)

£76.80

Description

These are the replacement items for the DNA detective kit: the same 5 tubes of pre-digested DNA with the 1 kb DNA ruler.
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