Slope culture of Bacillus subtilis on nutrient agar.
Detailed Description
This Gram-positive species produces a variety of extra-cellular enzymes (amylase, lipase and cellulase) which can be detected using an appropriate growth medium and test (i.e., starch agar, which is flooded with iodine in potassium iodide solution after growth; tributryn agar — clear zones show lipase activity or CMC agar stained with dilute Congo Red solution). It is susceptible to streptomycin. Grow on nutrient agar or broth (in broth, it will produce a pellicle on the surface of the liquid).
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